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	Provedor de dados PAB (9) BJM (1) Ciência Rural (1) Autor Monnerat,Rose Gomes (11) Martins,Érica Soares (4) Falcão,Rosana (3) Gomes,Ana Cristina Menezes Mendes (3) Gander,Eugen Silvano (2) Martins,Erica Soares (2) Praça,Lílian Botelho (2) Queiroz,Paulo Roberto (2) Santos,Roseane Cavalcanti (2) Soares,Carlos Marcelo Silveira (2) Arrigoni,Enrico de Beni (1) Baldani,José Ivo (1) Batista,Andréa Cardoso (1) Berry,Colin (1) Blum,Luiz Eduardo Bassay (1) Boas,Gislayne Trindade Vilas (1) Boas,Laurival Vilas (1) Bravo,Alejandra (1) Castro,Marcelo Tavares de (1) Constanski,Kelly Christiane (1) Mais... Palavra-chave Controle biológico (3) Anthonomus grandis (2) Diatraea saccharalis (2) Gossypium hirsutum (2) Proteínas Cry (2) Abscission (1) Aedes aegypti (1) Aleyrodidae (1) Animal feeding (1) Animal tissues (1) Bacillus thuringiensis kurstaki (1) Bactérias entomopatogênicas (1) Biocontrol (1) Bioinsecticide (1) Bioinseticidas (1) Biological control (1) Biopesticida (1) Combate às pragas (1) Commodities (1) Cristais protéicos (1) Mais... Tipo do documento Info:eu-repo/semantics/article (10) Info:eu-repo/semantics/report (1) Ano 2018 (1) 2016 (2) 2015 (1) 2012 (1) 2007 (1) 2005 (2) 2004 (1) 2003 (1) 2002 (1) Mais... País Brazil (11) Idioma Inglês (7) Português (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 11 Primeira ... 1 ... Última A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae) BJM Gitahy,Patrícia de Medeiros; Souza,Marlene Teixeira de; Monnerat,Rose Gomes; Arrigoni,Enrico de Beni; Baldani,José Ivo. The control of the major sugarcane pest, Diatraea saccharalis, is limited by the stem location of the caterpillar. As part of a long-term project towards the development of an alternative and efficient delivery system of Cry proteins to control the sugarcane borer, the current work describes the selection and characterization of a Brazilian B. thuringiensis strain with prominent activity towards D. saccharalis. Strain S76 was eleven-fold more active than the HD-1 Lepidoptera-standard strain, as estimated by the LC50 of 13.06 µg/L and 143.88 µg/L, respectively. We observed bipiramidal and cuboidal crystals similar to those found in other B. thuringiensis strains with entomopathogenic activity against Lepidoptera and Diptera. In addition, smaller and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Bacillus thuringiensis kurstaki; Cry toxins; Spherical crystal; Diatraea saccharalis. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300028 Caenorhabditis elegans as an indicator of toxicity of Bacillus thuringiensis strains to Meloidogyne incognita race 3 Ciência Rural Montalvão,Sandro Coelho Linhares; Castro,Marcelo Tavares de; Soares,Carlos Marcelo Silveira; Blum,Luiz Eduardo Bassay; Monnerat,Rose Gomes. ABSTRACT: The cotton plant (Gossypium hirsutum) is affected by several diseases of economic importance, among them root-knot nematode (Meloidogyne incognita races 3 and 4). Methods to control this disease include the application of nematicides, solarization, deep plowing, crop rotation and use of antagonistic microorganisms. Among species of Bacillus, there are strains that act as bioregulators and antagonists of several pathogens. Tests to identify these strains are hampered by the difficulty of obtaining large populations of the pathogen and by the time of execution of the in vivo tests that should be conducted for about 90 days. The objective of this research was to compare the toxicity of B. thuringiensis strains to two nematodes, M. incognita and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Biocontrol; Phytonematode; Root-knot nematode; Rhizobacteria. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700151 Cholesterol oxidase interference on the emergence and viability of cotton boll weevil larvae PAB Santos,Roseane Cavalcanti; Monnerat,Rose Gomes; Sá,Maria de Fátima Grossi de; Cordeiro,Célia Maria Torres; Gomes,Ana Cristina; Gander,Eugen Silvano. The aim of this work was to evaluate the influence of the enzyme cholesterol oxidase (Coase) on emergence and viability of larvae of the cotton boll weevil (Anthonomus grandis Boheman, 1843). A series of bioassays was performed with eggs and neonate larvae exposed to different enzyme concentrations in artificial diet. Larval survival was affected at all enzyme concentrations tested, and the six-day LD50 was 53 mug/mL (CI 95%: 43-59). Coase also interfered with hatching of larvae after eggs were floated for 15 min in Coase solution at different concentrations. Observations at the light and electronic microscopic level of midguts from larvae fed on artificial diet containing 53 mug/mL of Coase and collected at six days revealed highly vacuolated regions in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Anthonomus grandis; Insecta; Animal tissues; Epithelium; Intestinal histology. Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001100002 Estirpes de Bacillus thuringiensis efetivas contra insetos das ordens Lepidoptera, Coleoptera e Diptera PAB Praça,Lílian Botelho; Batista,Andréa Cardoso; Martins,Érica Soares; Siqueira,Claúdia Brod; Dias,Daniel Gerheim de Souza; Gomes,Ana Cristina Menezes Mendes; Falcão,Rosana; Monnerat,Rose Gomes. O objetivo deste trabalho foi selecionar entre 300 estirpes de Bacillus thuringiensis as efetivas simultaneamente contra larvas de Spodoptera frugiperda J.E. Smith e Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae), Anthonomus grandis Boheman (Coleoptera: Curculionidae), Aedes aegypti Linnaeus e Culex quinquefasciatus Say (Diptera: Culicidae). Foram selecionadas duas estirpes de B. thuringiensis, denominadas S234 e S997, que apresentaram atividade contra as três ordens de insetos. As estirpes foram caracterizadas por métodos morfológicos, bioquímicos e moleculares. As mesmas apresentaram duas proteínas principais de 130 e 65 kDa, produtos de reação em cadeia da polimerase de tamanho esperado para a detecção dos genes cry1Aa, cry1Ab, cry1Ac, cry1B e... Tipo: Info:eu-repo/semantics/report Palavras-chave: Larva; Controle biológico; Proteína; Reação em cadeia da polimerase; Combate às pragas; Biopesticida. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000100002 Identification of the B, Q, and native Brazilian biotypes of the Bemisia tabaci species complex using Scar markers PAB Queiroz,Paulo Roberto; Martins,Erica Soares; Klautau,Nazaré; Lima,Luzia; Praça,Lilian; Monnerat,Rose Gomes. Abstract: The objective of this work was to develop sequence-characterized amplified region (Scar) markers to identify the B, Q, and native Brazilian biotypes of the sweet potato whitefly [Bemisia tabaci (Hemiptera: Aleyrodidae)]. Random amplified polymorphic DNA (RAPD) amplification products, exclusive to the B and Brazilian biotypes, were selected after the analysis of 12,000 samples, in order to design a specific Scar primer set. The BT-B1 and BT-B3 Scar markers, used to detect the B biotype, produced PCR fragments of 850 and 582 bp, respectively. The BT-BR1 Scar marker, used to identify the Brazilian biotype, produced a PCR fragment of 700 bp. The Scar markers were tested against the Q biotype, and a flowchart was proposed to indicate the decision... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aleyrodidae; Commodities; Field samples; Molecular marker; Sweet potato whitefly.. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500555 Mechanical damage in cotton buds caused by the boll weevil PAB Santos,Roseane Cavalcanti; Marcellino,Lucília Helena; Monnerat,Rose Gomes; Gander,Eugen Silvano. The boll weevil (Anthonomus grandis Boheman) causes high levels of bud abscission in cotton plants due to feeding or oviposition punctures. It has been reported that abscission is mainly due to enzymes present in the insect's saliva, but mechanical damage could also contribute to square abscission. The objective of this paper was to undertake an analysis of the morphological damages caused by the insect in cotton squares using microscopy. Anthers and ovules are the main target of boll weevil feeding. The process initiates by perforation of young sepal and petal tissues and proceeds with subsequent alimentation on stamen and ovary leading to abscission of floral structures. Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Anthonomus grandis; Abscission; Animal feeding. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003001100015 Mitochondrial markers to distinguish Spodoptera frugiperda populations associated with corn and cotton crops PAB Queiroz,Paulo Roberto; Ramiro,Carolina Almeida; Martins,Érica Soares; Soberón,Mário; Bravo,Alejandra; Monnerat,Rose Gomes. Abstract: The objective of this work was to analyze the genetic variability of Spodoptera frugiperda (Lepidoptera: Noctuidae) populations collected from corn and cotton crops in Brazil. The samples were analyzed by DNA markers. The dendrogram produced by the 20 RAPD primers evaluated showed a correlation between genetic profile and feeding behavior. The analysis of the mitochondrial ND1 gene allowed identifying the insect populations in both crops, and, in corn, in several geographical regions. The presented strategy allows the identification of Spodoptera frugiperda populations associated with corn and cotton crops. Tipo: Info:eu-repo/semantics/article Palavras-chave: Gossypium hirsutum; Zea mays; Fall armyworm; Haplotypes; ND1 gene; RAPD.. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500692 Screening of Bacillus thuringiensis strains effective against mosquitoes PAB Monnerat,Rose Gomes; Dias,Daniel Gerhein Souza; Silva,Silvania Ferreira da; Martins,Erica Soares; Berry,Colin; Falcão,Rosana; Gomes,Ana Cristina Menezes Mendes; Praça,Lilian Botelho; Soares,Carlos Marcelo Silveira. The objective of this work was to evaluate 210 Bacillus thuringiensis strains against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective. These strains were isolated from different regions of Brazil and are stored in a Bacillus spp. collection at Embrapa Recursos Genéticos e Biotecnologia, Brasília, Brazil. The selected strains were characterized by morphological (microscopy), biochemical (SDS-PAGE 10%) and molecular (PCR) methods. Six B. thuringiensis strains were identified as mosquito-toxic after the selective bioassays. None of the strains produced the expected PCR products for detection of cry4, cry11 and cyt1A genes. These results indicate that the activity of mosquitocidal Brazilian strains are not related with Cry4, Cry11... Tipo: Info:eu-repo/semantics/article Palavras-chave: Culex quinquefasciatus; Aedes aegypti; Bioinsecticide. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005000200001 Seleção e caracterização de estirpes de Bacillus thuringiensis efetivas no controle da traça-das-crucíferas Plutella xylostella PAB Medeiros,Patrícia Teles; Ferreira,Márcio do Nascimento; Martins,Érica Soares; Gomes,Ana Cristina Menezes Mendes; Falcão,Rosana; Dias,José Manoel Cabral de Souza; Monnerat,Rose Gomes. O objetivo deste trabalho foi selecionar e caracterizar, no Banco de Germoplasma de Bacillus spp., da Empresa Brasileira de Pesquisa Agropecuária, as estirpes de Bacillus thuringiensis mais tóxicas à Plutella xylostella, por métodos morfológicos, bioquímicos e moleculares. Das 203 estirpes testadas, sete causaram 100% de mortalidade e foram semelhantes à estirpe padrão utilizada, B. thuringiensis subsp. kurstaki. Elas apresentaram proteínas de 130 kDa e 65 kDa, presença de genes cry1 e cry2 e cristais bipiramidais, cubóides e redondos. As estirpes selecionadas oferecem novas perspectivas de controle de P. xylostella. Tipo: Info:eu-repo/semantics/article Palavras-chave: Cristais protéicos; Bactérias entomopatogênicas; Bioinseticidas. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001100014 Seleção e caracterização de estirpes de Bacillus thuringiensis eficientes contra a Diatraea saccharalis (Lepidoptera: Crambidae) PAB Macedo,Cristina Lima de; Martins,Érica Soares; Macedo,Leonardo Lima Pepino de; Santos,Aldaléia Carmo dos; Praça,Lílian Botelho; Góis,Luiz Avelar Brandão de; Monnerat,Rose Gomes. O objetivo deste trabalho foi selecionar e caracterizar estirpes nativas de Bacillus thuringiensis tóxicas a Diatraea saccharalis (Lepidoptera: Crambidae). Cento e seis estirpes pertencentes ao Banco de Bactérias de Invertebrados, da Embrapa Recursos Genéticos e Biotecnologia, foram testadas quanto à toxicidade a D. saccharalis, e, as mais tóxicas, caracterizadas por métodos bioquímicos e moleculares. Das 106 estirpes testadas, 16 causaram 100% de mortalidade em 24 horas. As três estirpes mais tóxicas apresentaram concentração letal média entre 8 e 43 ng cm-2. O perfil proteico das 16 estirpes mostrou a presença de proteínas de 130 e 65 kDa, e a caracterização molecular mostrou a presença dos genes tipo cry1 e cry2: cry1Aa, cry1Ab, cry1Ac e cry2Aa. A... Tipo: Info:eu-repo/semantics/article Palavras-chave: Diatraea saccharalis; Controle biológico; Ensaio de ligação; Proteínas Cry. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012001200012 Seleção e caracterização molecular de isolados de Bacillus thuringiensis para o controle de Spodoptera spp. PAB Constanski,Kelly Christiane; Zorzetti,Janaina; Boas,Gislayne Trindade Vilas; Ricieto,Ana Paula Scaramal; Fazion,Fernanda Aparecida Pires; Boas,Laurival Vilas; Monnerat,Rose Gomes; Neves,Pedro Manuel Oliveira Janeiro. Resumo:O objetivo deste trabalho foi selecionar e caracterizar molecularmente isolados de Bacillus thuringiensistóxicos a Spodoptera eridaniaeS. frugiperda. Trinta e quatro isolados foram submetidos ao bioensaio, dos quais três foram selecionados e usados para a estimativa da CL50. Os isolados selecionados não diferiram da linhagem padrão HD-1. Na caracterização molecular, identificou-se a presença dos genes cry1 e cry2, nos isolados BR37 e BR94, e dos genes cry4A, cry4B, cry10, cry11 e cyt1 no isolado BR58, o que confirmou o perfil proteico obtido de 130, 70 e 65 kDa. Foram identificados cristais bipiramidais e esféricos. O isolado BR58, apesar de não conter os genes relacionados à toxicidade a Lepidoptera, causa mortalidade em ambas as espécies Tipo: Info:eu-repo/semantics/article Palavras-chave: Spodoptera eridania; Spodoptera frugiperda; Controle biológico; Genes cry; Proteínas Cry. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000800730 Registros recuperados: 11 Primeira ... 1 ... Última

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